Coding
Part:BBa_K3317004:Design
Designed by: erick hernandez Group: iGEM19_UANL (2019-10-16)
Vanillate-responsive transcriptional repressor (VanR)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 384
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Adapted by Kunjapur, A. M et.al 2018, enhanced by Meyer A. et.al 2018
Source
VanR is a functional vanillate sensor from Caulobacter crescentus adapted for E. coli
References
Meyer, A. J., Segall-Shapiro, T. H., Glassey, E., Zhang, J., & Voigt, C. A. (2019). Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nature chemical biology, 15(2), 196.
Kunjapur, A. M., & Prather, K. L. (2018). Development of a vanillate biosensor for the vanillin biosynthesis pathway in E. coli. bioRxiv, 375287.