Coding

Part:BBa_K3317004:Design

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-16)


Vanillate-responsive transcriptional repressor (VanR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adapted by Kunjapur, A. M et.al 2018, enhanced by Meyer A. et.al 2018


Source

VanR is a functional vanillate sensor from Caulobacter crescentus adapted for E. coli

References

Meyer, A. J., Segall-Shapiro, T. H., Glassey, E., Zhang, J., & Voigt, C. A. (2019). Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nature chemical biology, 15(2), 196.

Kunjapur, A. M., & Prather, K. L. (2018). Development of a vanillate biosensor for the vanillin biosynthesis pathway in E. coli. bioRxiv, 375287.